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检测工业葡萄糖含量的方法?
来源:http://www.jnjdbc.com 日期:2021-05-17 发布人:adminputaotang
1.原理工业葡萄糖在葡萄糖氧化酶的作用下产生葡萄糖酸和过氧化氢,过氧化氢在过氧化物酶的作用下使邻联甲苯胺生成蓝色物质,此有色物质在625nm 波长下与葡萄糖浓度成正比。通过测定蓝色物质的吸光度可计算样品中工业葡萄糖的含量。
1. Principle: Industrial glucose produces gluconic acid and hydrogen peroxide under the action of glucose oxidase. Hydrogen peroxide makes o-toluidine produce blue substance under the action of peroxidase. This colored substance is proportional to the concentration of glucose at 625nm. The content of industrial glucose in the sample can be calculated by measuring the absorbance of blue substance.
2.适用范围适用于谷类、乳类、饮料、酒类等食物样品和血液样品。
2. The scope of application is applicable to cereal, milk, beverage, alcohol and other food samples and blood samples.
             无水葡萄糖
3.计算根据吸光度值求出工业葡萄糖标准曲线的回归方程,采用插入法求出样品测定管中葡萄糖含量,再根据稀释定容体积和称样量,计算出样品中工业葡萄糖含量。
3. Calculate the regression equation of the industrial glucose standard curve according to the absorbance value, calculate the glucose content in the sample determination tube by the insertion method, and then calculate the industrial glucose content in the sample according to the diluted constant volume and the sample weight.
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